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α rab9a  (Proteintech)


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    Structured Review

    Proteintech α rab9a
    α Rab9a, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/%CE%B1+rab9a/RAB9A+Antibody/bio_rxiv__2022__09__25__509379-175-57-58
    Average 93 stars, based on 8 article reviews
    α rab9a - by Bioz Stars, 2026-09
    93/100 stars

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    Immunofluorescence:

    Article Title: EGFR-initiated endocytosis of Wnt9a and Fzd9b is required for β-catenin signaling
    Article Snippet: .. For immunofluorescence, cells were prepared and fixed as above, and incubated in blocking buffer (2% BSA in PBS ++ ) for 60 minutes at room temperature, and in primary antibody (α-tRFP, [mKate antibody, Evrogen, AB233], α-FLAG, [Sigma, F1804], α-V5, [Cell Signaling Technologies, 13202S], α-Fzd9b , α-Wnt9a , α-Rab5, [Cell Signaling Technologies, 3547S], α-Rab7, [Cell Signaling Technologies, 9367S], α-Rab9a, [Proteintech, 11420-1-AP], α-Gpr94, [Cell Signaling Technologies, 20292], α-LAMP1, [Invitrogen, MA5-29385]) diluted 1:50-1:250 in blocking buffer overnight at 4°C. .. Cells were washed three times in PBS ++ , incubated in secondary antibody (1:500 for each, Goat-α-mouse Alexa 488 [cat# A11001, lot# 2189178], goat-α-rabbit Alexa 488 [cat# 4050-30, lot# e1317 NC39], goat-α-mouse Alexa 555 [cat# 1030-32, lot# E2518 ZA08E], goat-α-rabbit Alexa 647 [cat# 4050-31, lot# E1519 S109Z], Southern Biotech) diluted in blocking buffer for 60 minutes at room temperature, washed three times in PBS ++ , and mounted in vectashield mounting medium (with or without DAPI).

    Incubation:

    Article Title: EGFR-initiated endocytosis of Wnt9a and Fzd9b is required for β-catenin signaling
    Article Snippet: .. For immunofluorescence, cells were prepared and fixed as above, and incubated in blocking buffer (2% BSA in PBS ++ ) for 60 minutes at room temperature, and in primary antibody (α-tRFP, [mKate antibody, Evrogen, AB233], α-FLAG, [Sigma, F1804], α-V5, [Cell Signaling Technologies, 13202S], α-Fzd9b , α-Wnt9a , α-Rab5, [Cell Signaling Technologies, 3547S], α-Rab7, [Cell Signaling Technologies, 9367S], α-Rab9a, [Proteintech, 11420-1-AP], α-Gpr94, [Cell Signaling Technologies, 20292], α-LAMP1, [Invitrogen, MA5-29385]) diluted 1:50-1:250 in blocking buffer overnight at 4°C. .. Cells were washed three times in PBS ++ , incubated in secondary antibody (1:500 for each, Goat-α-mouse Alexa 488 [cat# A11001, lot# 2189178], goat-α-rabbit Alexa 488 [cat# 4050-30, lot# e1317 NC39], goat-α-mouse Alexa 555 [cat# 1030-32, lot# E2518 ZA08E], goat-α-rabbit Alexa 647 [cat# 4050-31, lot# E1519 S109Z], Southern Biotech) diluted in blocking buffer for 60 minutes at room temperature, washed three times in PBS ++ , and mounted in vectashield mounting medium (with or without DAPI).

    Blocking Assay:

    Article Title: EGFR-initiated endocytosis of Wnt9a and Fzd9b is required for β-catenin signaling
    Article Snippet: .. For immunofluorescence, cells were prepared and fixed as above, and incubated in blocking buffer (2% BSA in PBS ++ ) for 60 minutes at room temperature, and in primary antibody (α-tRFP, [mKate antibody, Evrogen, AB233], α-FLAG, [Sigma, F1804], α-V5, [Cell Signaling Technologies, 13202S], α-Fzd9b , α-Wnt9a , α-Rab5, [Cell Signaling Technologies, 3547S], α-Rab7, [Cell Signaling Technologies, 9367S], α-Rab9a, [Proteintech, 11420-1-AP], α-Gpr94, [Cell Signaling Technologies, 20292], α-LAMP1, [Invitrogen, MA5-29385]) diluted 1:50-1:250 in blocking buffer overnight at 4°C. .. Cells were washed three times in PBS ++ , incubated in secondary antibody (1:500 for each, Goat-α-mouse Alexa 488 [cat# A11001, lot# 2189178], goat-α-rabbit Alexa 488 [cat# 4050-30, lot# e1317 NC39], goat-α-mouse Alexa 555 [cat# 1030-32, lot# E2518 ZA08E], goat-α-rabbit Alexa 647 [cat# 4050-31, lot# E1519 S109Z], Southern Biotech) diluted in blocking buffer for 60 minutes at room temperature, washed three times in PBS ++ , and mounted in vectashield mounting medium (with or without DAPI).



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    α rab9a  (Proteintech)
    93
    Proteintech α rab9a
    α Rab9a, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/%CE%B1+rab9a/RAB9A+Antibody/bio_rxiv__2022__09__25__509379-175-57-58
    Average 93 stars, based on 1 article reviews
    α rab9a - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier

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